منابع مشابه
Centrifuge Experiments with the Virus of Vaccinia
Centrifuge experiments have been carried out with cell-free, active filtrates of vaccinia virus. The experiments have shown that the virus can be concentrated by this method, even in filtrates which have been subjected to prolonged preliminary centrifugation to throw down any inert particles which may have been present in the original filtrate. This fact, together with the knowledge that the vi...
متن کاملVaccinia virus motility.
Vaccinia virus (VV), the virus smallpox vaccine, replicates in the cytoplasm of infected cells. The intracellular movement of this large virus would be inefficient without specific transport mechanisms; therefore, VV uses microtubules for movement during both entry and egress. In addition, the dissemination of virus from infected cells to adjacent cells is promoted by the polymerization of acti...
متن کاملVaccinia virus transcription.
Vaccinia virus replication takes place in the cytoplasm of the host cell. The nearly 200 kbp genome owes part of its complexity to encoding most of the proteins involved in genome and mRNA synthesis. The multisubunit vaccinia virus RNA polymerase requires a separate set of virus-encoded proteins for the transcription of the early, intermediate and late classes of genes. Cell fractionation studi...
متن کاملVaccinia Virus: Isolation
more. Virus yield, which decreased only when the drug was added early in infection, was reduced several 100fold by 80 tLM aphidicolin. Viral inhibition was reversed by the suspension of the infected cells in drug-free medium. DNA synthesis in uninfected cells was reduced about 10-fold by 1 ,uM aphidicolin. In infected cells, aphidicolin concentrations over 10 ,uM were needed to reduce DNA synth...
متن کاملUncoating of vaccinia virus.
Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against DNase digestion. It is suggested that viral...
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ژورنال
عنوان ژورنال: Journal of Hygiene
سال: 1932
ISSN: 0022-1724
DOI: 10.1017/s0022172400017824